
产品名称:Anti-HA tag 小鼠单抗偶联Agarose Beads
货号: AT0079
品牌:Engibody
(对应关键词:anti-HA affinity gel, HA亲和胶, HA 抗体beads, HA 抗体agarose)
抗 HA 标签小鼠单克隆抗体偶联琼脂糖珠,适用于HA融合蛋白的免疫沉淀 (IP)、共免疫沉淀 (Co-IP)、HA 融合蛋白纯化。
产品描述
抗 HA 标签小鼠单克隆抗体偶联琼脂糖珠
反应性
所有表达HA标签融合蛋白的系统均可作为样本以便富集捕获HA融合蛋白(包含细菌表达系统,酵母表达系统,昆虫和哺乳动物细胞表达系统及体外无细胞转录翻译系统)
适用于HA融合蛋白的免疫沉淀 (IP)、共免疫沉淀 (Co-IP)、HA 融合蛋白纯化。
验证过的应用
免疫沉淀 (IP)、共免疫沉淀 (Co-IP)、HA 融合蛋白纯化
在 IP 中,40 µL 珠子悬浮液(20 µL 包装珠子)用于约 500-1000 µL 细胞裂解液。最佳稀释度/浓度应由最终用户确定。
特异性
抗 HA-Tag 小鼠单克隆抗体偶联琼脂糖珠,可识别天然和变性还原形式的 HA(血凝素)标记蛋白,并与大肠杆菌中表达的 N 或 C 末端 HA 标记融合蛋白反应或在细胞中。
免疫原
这种单克隆抗体是通过使用与人流感病毒血凝素 (HA) 的氨基酸残基 98-106 (YPYDVPDYA) 对应的合成肽(称为 HA-tag)对小鼠进行免疫接种而产生的,该肽与 KLH 偶联。
克隆性
单克隆抗体,克隆号:6H2
偶联抗体同型对照
小鼠 IgG1
产品形式
该产品以 10 mM 磷酸盐缓冲盐水 (PBS)、pH 7.4、0.02% (w/v) 防腐剂的 50% 悬浮液形式提供。
存放说明
4℃保存,不可冷冻
珠子大小:
~ 90 µm(交联 8% 琼脂糖珠子)
结合能力:
每 1 mL 凝胶浆液可以结合富集>1mg HA 标记的融合蛋白。
Description
Anti- HA tag mouse monoclonal antibody conjugated Agarose Beads
Reactivity
Species independent
Tested applications
Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), HA fusion protein purification
In IP, 40 µL of beads suspension (20µL of packed beads) for ~500-1000 µL of cell lysate. Optimal dilutions/concentrations should be determined by the end user.
Specificity
anti-HA-Tag mouse monoclonal antibody Conjugated Agarose beads, recognizes native as well as denatured-reduced forms of the HA (hemagglutinin)-tagged proteins and is reactive with N-or C-terminal HA-tagged fusion proteins expressed in E.coli or in cells.
Immunogen
This monoclonal antibody is produced by immunizing mice with a synthetic peptide corresponding to amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH.
Clonality
Monoclonal antibody, clone number: 6H2
Isotype
Mouse IgG1
Form
The product is supplied as a 50% suspension in 10 mM phosphate buffered saline (PBS), pH 7.4, 0.02% (w/v) sodium azide as preservative.
Storage instruction
Stored at 4 °C, do not freeze
Bead size:
~ 90 µm (cross-linked 8% agarose beads)
Binding capacity:
>1mg of HA-tagged fusion protein per 1 mL of gel slurry.