Anti-His小鼠单克隆抗体偶联Agarose Beads

产品名称：Anti-His小鼠单克隆抗体偶联Agarose Beads

货号：AT0084      

品牌：Engibody

（对应关键词：His 亲和胶，His gel, His affinity gel, His beads, his agarose）

Anti-6X His 琼脂糖珠（亲和凝胶）是一种小鼠单克隆抗体，通过酰肼键共价连接到琼脂糖珠上。 该抗体在融合蛋白的 N 端、C 端和内部位置结合 6X His 标签。用于his蛋白的IP、CoIP及his蛋白的精细纯化。

产品描述

抗 6X His 标签小鼠单克隆抗体偶联琼脂糖珠

反应性

所有表达His标签融合蛋白的系统均可作为样本以便富集捕获his融合蛋白（包含细菌表达系统，酵母表达系统，昆虫和哺乳动物细胞表达系统及体外无细胞转录翻译系统）

验证过的应用

免疫沉淀 (IP)、共免疫沉淀 (Co-IP)、His 标签融合蛋白纯化

在 IP 中，40 µL 珠浆（20 µL 沉降珠子）用于约 500-1000 µL 细胞裂解液。

最佳稀释度/浓度应由最终用户确定。

特异性

该抗体可检测并捕获在细胞或大肠杆菌中过表达的 6X His-Tagged 融合蛋白。

免疫原

这种单克隆抗体是通过用含有 6X His 的合成肽（KLH 偶联）对小鼠进行免疫接种而产生的。

克隆性

单克隆抗体，克隆号：2B6

偶联抗体同型对照

小鼠 IgG1

形式

该产品以 10 mM 磷酸盐缓冲盐水 (PBS)、pH 7.4、0.02% (w/v) 防腐剂的 50% 悬浮液形式提供。

存放说明

4℃保存，不可冷冻

珠子大小：

~ 90 µm（交联 8% 琼脂糖珠子）

结合能力：

>1 mg 6X His-tagged 融合蛋白/1 mL 珠浆。

Description

Anti-6X His tag mouse monoclonal antibody conjugated Agarose Beads

Reactivity

Species independent

Tested applications

Immunoprecipitation (IP), Co-Immunoprecipitation (Co-IP), His tagged fusion protein purification

In IP, 40 µL of beads slurry (20µL of settled beads) for ~500-1000 µL of cell lysate.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

The antibody detects and captures 6X His -Tagged fusion proteins overexpressed in cells or E. coli.

Immunogen

This monoclonal antibody is produced by immunizing mice with a synthetic peptide (KLH-coupled) containing 6X His.

Clonality

Monoclonal antibody, clone number: 2B6

Isotype

Mouse IgG1

Form

The product is supplied as a 50% suspension in 10 mM phosphate buffered saline (PBS), pH 7.4, 0.02% (w/v) sodium azide as preservative.

Storage instruction

Stored at 4 °C, do not freeze

Bead size:

~ 90 µm (cross-linked 8% agarose beads)

Binding capacity:

>1 mg of 6X His -tagged fusion protein per 1 mL of beads slurry.