
产品名称:HA-Catch-Aga™ Anti-HA tag羊驼纳米抗体偶联Agarose Beads
货号: AT1763
品牌:Engibody
(对应关键词:HA trap , HA trap agarose, HA羊驼纳米抗体)
产品描述
HA-Catch-Aga™ Anti-HA 标签纳米抗体(来自羊驼 VHH,单域抗体)偶联琼脂糖珠
反应性
该产品是对带有HA标签的蛋白质进行IP/CoIP/ChIP的有用工具,它可以识别放置在融合蛋白N端、C端或内部位点的HA标签。
所有表达HA标签融合蛋白的系统均可作为样本以便富集捕获HA融合蛋白(包含细菌表达系统,酵母表达系统,昆虫和哺乳动物细胞表达系统及体外无细胞转录翻译系统)
验证过的应用
免疫沉淀(IP),
免疫共沉淀 (Co-IP),
染色质免疫沉淀 (ChIP)
RNA结合蛋白免疫沉淀(RIP)
酶测定
质谱
亲和纯化
在 IP 中,25-50 μL 的珠子悬浮液用于约 500-1000 μL 的粗总细胞裂解液溶液。
最佳稀释度/浓度应由最终用户确定。
特异性
该抗体可捕获在细胞或大肠杆菌中外源表达的 HA 标记蛋白。
免疫原
这种纳米抗体是由带有 HA 标签融合蛋白免疫羊驼制成的。
形式
50%珠浆
琼脂糖珠偶联抗 HA 纳米抗体,产品以 10 mM PBS、pH 7.4 和 0.02% (w/v) 防腐剂提供。
存放说明
储存于 4 °C。不要冻结
珠子直径
~ 90 微米
结合能力
~1mg HA 标记蛋白/ml 琼脂糖珠悬浮液
Description
HA-Catch-Aga™ Anti-HA tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Agarose Beads
Reactivity
This product is useful tool for IP/CoIP/ChIP of HA-tagged proteins, and it recognizes HA-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.
Tested applications
Immunoprecipitation (IP),
Co-Immunoprecipitation (Co-IP),
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Enzyme assays
Mass spectrometry
Affinity purification
In IP, 25-50 µL of beads suspension for ~500-1000 µL of crude total cell lysate solution.
Optimal dilutions/concentrations should be determined by the end user.
Specificity
This antibody captures HA-tagged proteins exogenously expressed in cells or E. coli.
Immunogen
This nanobody is produced from alpaca with a HA tagged protein.
Form
50% beads slurry
Agarose beads Conjugated anti-HA nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative.
Storage instruction
Stored at 4 °C. Do not freeze
Beads Diameter
~ 90 µm
Binding Capacity
~1mg HA tagged protein /ml beads suspension