
产品名称:mCherry-Catch-Mag™ Anti-mCherry羊驼纳米抗体偶联磁珠
货号:AT1775
品牌:Engibody
对应关键词: mCherry trap磁珠, mCherry磁珠, mCherry羊驼纳米抗体磁珠
产品描述
mCherry-Catch-Mag™ 抗 mCherry 标签纳米抗体(来自 alpaca VHH,单域抗体)偶联磁珠
反应性
该产品是mCherry-tagged蛋白IP/CoIP/ChIP的高效工具,它可以识别位于重组蛋白N端、C端的mCherry-tags。
所有表达mCherry标签融合蛋白的系统均可作为样本以便富集捕获mCherry融合蛋白(包含细菌表达系统,酵母表达系统,昆虫和哺乳动物细胞表达系统及体外无细胞转录翻译系统)
验证过的应用
免疫沉淀(IP),
免疫共沉淀 (Co-IP),
染色质免疫沉淀 (ChIP)
RNA结合蛋白免疫沉淀(RIP)
酶测定
质谱
亲和纯化
在 IP 中,25-50 μL 的珠子悬浮液用于约 500-1000 μL 的粗总细胞裂解液溶液。
最佳稀释度/浓度应由最终用户确定。
特异性
该抗体可检测在细胞或大肠杆菌中外源表达的 mCherry 标签蛋白。该抗体不能检测 GFP 标记的蛋白质。
免疫原
这种纳米抗体是由具有 mCherry 蛋白的羊驼制成的。
形式
磁珠偶联抗 mCherry 纳米抗体,该产品以 10 mM PBS、pH 7.4 和 0.02% (w/v) 防腐剂提供。
存放说明
储存于 4 °C。不要冻结
磁珠直径
~ 40 微米
结合能力
~1mg mCherry 蛋白/ml 珠子悬浮液
Description
mCherry-Catch-Mag™ Anti-mCherry tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Magnetic Beads
Reactivity
This product is useful tool for IP/CoIP/ChIP of mCherry-tagged proteins, and it recognizes mCherry-tags placed at N-terminal, C-terminal of the recombinant proteins.
Tested applications
Immunoprecipitation (IP),
Co-Immunoprecipitation (Co-IP),
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Enzyme assays
Mass spectrometry
Affinity purification
In IP, 25-50 µL of beads suspension for ~500-1000 µL of crude total cell lysate solution.
Optimal dilutions/concentrations should be determined by the end user.
Specificity
This antibody detects mCherry-tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect GFP-tagged proteins.
Immunogen
This nanobody is produced from alpaca with a mCherry protein.
Form
Magnetic beads Conjugated anti-mCherry nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative.
Storage instruction
Stored at 4 °C. Do not freeze
Beads Diameter
~ 40 µm
Binding Capacity
~1mg mCherry protein /ml beads suspension