
产品名称:Flag-Catch-Mag™ Anti-DYKDDDDK (FLAG) 羊驼纳米抗体偶联磁珠
货号:AT1770
品牌:Engibody
对应关键词:Flag trap磁珠,Flag羊驼纳米抗体磁珠
描述
Flag-Catch-Mag™ Anti-Flag 标签纳米抗体(来自羊驼 VHH,单域抗体)偶联磁珠
反应性
该产品是用于flag标签融合蛋白的 IP/CoIP/ChIP 的高效工具,它可以识别在融合蛋白 N 端、C 端或内部位点的标签。
所有表达flag标签融合蛋白的系统均可作为样本以便富集捕获flag融合蛋白(包含细菌表达系统,酵母表达系统,昆虫和哺乳动物细胞表达系统及体外无细胞转录翻译系统)
验证过的应用
免疫沉淀(IP),
免疫共沉淀 (Co-IP),
染色质免疫沉淀 (ChIP)
RNA结合蛋白免疫沉淀(RIP)
酶测定
质谱
亲和纯化
在 IP 中,25-50 μL 的磁珠悬液用于约 500-1000 μL 的粗总细胞裂解液溶液。
最佳稀释度/浓度应由最终用户确定。
特异性
该抗体可检测在细胞或大肠杆菌中外源表达的带有 Flag 标签的蛋白质。该抗体还可检测 3*Flag-tagged 蛋白质。
免疫原
这种纳米抗体是由带有标记蛋白的羊驼制成的。
形式
磁珠共偶联抗标记纳米抗体,该产品以 10 mM PBS、pH 7.4 和 0.02% (w/v) 防腐剂提供。
存放说明
储存于 4 °C。不要冻结
磁珠直径
~ 40 微米
结合能力
~1mg 标记蛋白/ml 磁珠悬液
Description
Flag-Catch-Mag™ Anti-Flag tag nanobody (from alpaca VHH, Single Domain antibody) conjugated Magnetic Beads
Reactivity
This product is useful tool for IP/CoIP/ChIP of Flag-tagged proteins, and it recognizes Flag-tags placed at N-terminal, C-terminal, or internal site of the fusion protein.
Tested applications
Immunoprecipitation (IP),
Co-Immunoprecipitation (Co-IP),
Chromatin Immunoprecipitation (ChIP)
RNA Binding Protein Immunoprecipitation (RIP)
Enzyme assays
Mass spectrometry
Affinity purification
In IP, 25-50 µL of beads suspension for ~500-1000 µL of crude total cell lysate solution.
Optimal dilutions/concentrations should be determined by the end user.
Specificity
This antibody detects Flag-tagged proteins exogenously expressed in cells or E. coli. This antibody also detects 3*Flag-tagged proteins.
Immunogen
This nanobody is produced from alpaca with a flag tagged protein.
Form
Magnetic beads Conjugated anti-Flag nanobody, the product is supplied in 10 mM PBS, pH 7.4, and 0.02% (w/v) sodium azide as preservative.
Storage instruction
Stored at 4 °C. Do not freeze
Beads Diameter
~ 40 µm
Binding Capacity
~1mg Flag tagged protein /ml beads suspension