ECL发光液

产品名称：ECL Pico-Detection Western Blotting Substrate，ECL发光液

货号：IF6747

品牌：Engibody

（对应关键词：ECL发光液, SuperSignal, West Pico,  Immobilon）

ECL化学发光液用于检测直接或间接标记辣根过氧化物酶（HRP）的抗体及其关联的抗原。其检测原理如图1所示，蛋白质在电泳后转移到印迹膜上，以一抗及HRP标记的二抗结合膜上的目的蛋白。洗膜后用ECL化学发光液室温孵育膜数分钟，目的蛋白就可在X光胶片上获得清晰条带。

ECL Pico-Detection采用创新的ECL化学发光底物，极大地提高检测灵敏度和延长发光时间。本公司的ECL化学发光液即适用于X光胶片上曝光成像，也适用于CCD照相机成像，操作及其方便，是WB检测的优秀试剂。

高灵敏度  ̶  ECL Pico-detection检测极限达到pg级蛋白质，ECL Femto-detection检测极限达到fg级蛋白质

更短的曝光时间  ̶ 产生相同信号只需更短的曝光时间

发光时间持续长  ̶  ECL Pico-detection可以持续4小时

节省抗体  ̶  抗体使用量只需常规试剂的1/10

ECL chemiluminescence solution is used to detect antibodies that directly or indirectly label horseradish peroxidase (HRP) and its associated antigens. The detection principle is shown in Figure 1. After electrophoresis, the protein is transferred to the imprinted membrane, and the target protein on the membrane is bound by the primary antibody and the HRP labeled secondary antibody. After washing the film, incubate the film with ECL chemiluminescence solution at room temperature for several minutes, and the target protein can obtain clear bands on the X-ray film.

Engibody Company adopts innovative ECL chemiluminescence substrate to greatly improve detection sensitivity and prolong luminescence time. ECL chemiluminescence solution of our company is not only suitable for exposure imaging on X-ray film, but also suitable for CCD chemiluminescence imager imaging. It is easy to operate and is the preferred reagent for WB detection.

Highlights

Stronger signal than other chemiluminescent substrates for film imaging

Load less sample – conserve precious sample

Detect low pg amounts of protein – for detecting low abundance proteins

Use up to ten-times less antibody – conserve precious antibodies

Long lasting signal – carry out multiple exposures at your convenience without signal decay

Stable signal – carry out longer exposures to detect low abundance proteins