Anti-GAPDH小鼠单克隆抗体

产品名称：Anti-GAPDH小鼠单克隆抗体 – 内参抗体

货号： AT0002  

品牌：Engibody

简介：亲和力高，特异性好，条带锐利清晰。

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression, and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. This antibody recognizes the monomer (36 kDa).

Description

Mouse monoclonal antibody to GAPDH - Loading Control

Reactivity

Human, mouse, rat

Tested applications

WB : 1/2000-1/10000. Predicted molecular weight: 36 kDa.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects endogenous levels of GAPDH.

Immunogen

a synthetic peptide corresponding to residues near the carboxy terminus of human GAPDH

Clonality

Monoclonal, clone number: 3G7

Isotype

Mouse IgG1

Form

Liquid, 100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Database links

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

甘油醛-3-磷酸脱氢酶（GAPDH）在糖酵解过程中催化甘油醛-3-3-磷酸酯的磷酸化。虽然GAPDH在不同组织间表达不同，但被认为是一种组成性表达的管家蛋白。由于这个原因，GAPDH mRNA和蛋白水平通常在实验中作为对照来测量，以量化其他靶点表达的特定变化。最近的研究阐明了GAPDH在凋亡、基因表达和核转运中的作用。GAPDH也可能在神经退行性疾病如亨廷顿病和阿尔茨海默病中发挥作用。

GAPDH是由四个30-40kDa亚基组成的146kDa四聚体。该抗体识别单体（36kDa）。

描述

小鼠GAPDH单克隆抗体-WB内参抗体

反应性

人、小鼠、大鼠

已测试的应用

WB:1/2000-1/10000。预测分子量：36 kDa。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

该抗体检测内源性GAPDH水平。

免疫原

与人GAPDH羧基末端附近残基对应的合成肽

克隆性

单克隆，克隆号：3G7

抗体亚型

小鼠IgG1

类型

液体，100μg，1mg/mL

小鼠单克隆免疫球蛋白，磷酸盐缓冲盐水（PBS），pH 7.3（+/-0.1），50%甘油，0.1mg/mL BSA（IgG，无蛋白酶）作为稳定剂，0.02%抑菌剂作为防腐剂。

存储说明

储存在-20°C下，避免冷冻/解冻循环。

数据库链接

SwissProt:P04406人类

SwissProt:P16858小鼠

SwissProt:P04797大鼠

All lanes: Anti-GAPDH antibody at 1:5000

Lane 1: HeLa whole cell lysate

Lane 2: HEK293 cell lysate

Lane 3: Jurkat cell lysate

Predicted band size: 36 kDa

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Western Blot: GAPDH Antibody - WB detection of GAPDH protein in lysates of Mouse cell lines (A) NIH 3T3 (B) RAW 264.7 using GAPDH antibody at a dilution 1:10000.

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)