Anti-beta Tubulin 小鼠单克隆抗体

简要描述：beta tubulin是常用的WB实验内参抗体，分子量为55kDa

产品型号：AT0003
厂商性质：代理商
更新时间：2023-03-23
访问量：60

产品咨询联系我们

详情介绍

产品名称：Anti-beta Tubulin 小鼠单克隆抗体 – 内参抗体

货号： AT0003

品牌：Engibody

简介：

亲和力高，特异性好，条带锐利清晰。分子量为55 kDa

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. γ-tubulin is required to nucleate polymerization of tubulin subunits to form microtubule polymers. Many cell movements are mediated by microtubule action, including the beating of cilia and flagella, cytoplasmic transport of membrane vesicles, chromosome alignment during meiosis/mitosis, and nerve-cell axon migration. These movements result from competitive microtubule polymerization and depolymerization or through the actions of microtubule motor proteins.

Description

Mouse monoclonal antibody to β-Tubulin - Loading Control

Reactivity

Human, mouse, rat, rabbit, monkey, cow, hamster.

Tested applications

WB : 1/2000-1/10000. Predicted molecular weight: 55 kDa.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects endogenous levels of beta Tubulin.

Immunogen

a synthetic peptide corresponding to the amino terminus of human β-tubulin.

Clonality

Monoclonal, clone number: 6C4

Isotype

Mouse IgG1

Form

Liquid, 100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle.

Database links

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

Host

Mouse

细胞骨架由三种类型的胞质纤维组成：微管、微丝（肌动蛋白丝）和中间丝。球状微管蛋白亚基构成微管构建块，α/β-微管蛋白异二聚体形成所有真核细胞共有的微管蛋白子基。γ-微管蛋白是微管蛋白亚基成核聚合形成微管聚合物所必需的。许多细胞运动由微管作用介导，包括纤毛和鞭毛的跳动、膜囊的细胞质运输、减数分裂/有丝分裂期间的染色体排列和神经细胞轴突迁移。这些运动源于竞争性微管聚合和解聚或通过微管运动蛋白的作用。

描述

β-Tubulin的小鼠单克隆抗体-WB实验内参

反应性

人，大鼠，小鼠，兔子，猴子，牛，仓鼠。

已测试的应用

WB:1/2000-1/10000。预测分子量：55 kDa。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

抗体检测内源性β-tubulin。

免疫原

一种合成肽，对应于人β-微管蛋白的氨基末端。

克隆性

单克隆，克隆号：6C4

抗体亚型

小鼠IgG1

类型

液体，100μg，1mg/mL

小鼠单克隆免疫球蛋白，磷酸盐缓冲盐水（PBS），pH 7.3（+/-0.1），50%甘油，0.1mg/mL BSA（IgG，无蛋白酶）作为稳定剂，0.02%抑菌剂作为防腐剂。

存储说明

储存在-20°C下，避免冷冻/解冻循环。

数据库链接编号

SwissProt:P07437人类

SwissProt:P99024小鼠

SwissProt:P69897大鼠

抗体来源宿主

小鼠

Western blot shows lysates of human brain (cerebellum) tissue, human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and mouse brain (stem) tissue. A specific band was detected for beta Tubulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions.