Anti-beta Actin兔单抗 – 内参抗体

产品名称：Anti-beta Actin兔单抗 – 内参抗体

货号： AT0009

品牌：Engibody

简介：

亲和力高，特异性好，条带锐利清晰。

Description

anti-β-Actin Rabbit monoclonal antibody

Reactivity

Mouse, Rat, Human

Tested applications

WB:1/1000-1/10000

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

Synthetic peptide derived from within residues 1 - 100 of Human beta Actin.

Clonality

Monoclonal, clone number: 9E6

Isotype

Rabbit IgG

Form

Liquid

Preservative: 0.02% Sodium Azide

Constituents: 1% BSA, PBS. pH 7.4

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

描述

抗β-actin蛋白兔单克隆抗体

识别物种

大鼠，小鼠，人类

已测试的应用实验

效价：1/1000-1/10000

尚未在其他应用中测试。

最佳稀释/浓度应由最终用户确定。

免疫原

人β肌动蛋白残基1-100内衍生的合成肽。

克隆性

单克隆，克隆号：9E6

抗体亚型

兔IgG

成分

液体

防腐剂：0.02%抑菌剂

缓冲液：1%BSA，PBS。pH 7.4

存储说明

-20°C储存。避免重复冷冻/解冻循环。

All lanes : Anti-beta Actin antibody - Loading Control (AT0009) at 1:5000

Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate

Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

Lysates/proteins at 30 µg per lane.

Secondary

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Performed under reducing conditions.

Predicted band size : 42 kDa

Observed band size : 42 kDa

Western Blot: Beta Actin Rabbit Monoclonal Antibody (AT0009)

Analysis of Beta Actin on different lysates using this antibody at 1:1000 dilution.

Lane 1: HEK293, Lane 2: Hela, Lane 3: PC-12, Lane 4: NIH/3T3