Anti-GAPDH兔单抗 – 内参抗体

产品名称：Anti-GAPDH兔单抗 – 内参抗体

货号： AT0010

品牌：Engibody

简介：

亲和力高，特异性好，条带锐利清晰。

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.

Description

anti-GAPDH Rabbit monoclonal antibody

Reactivity

Mouse, Rat, Human

Tested applications

WB: 1/2000-1/10000

Not yet tested in other applications.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

Full length native protein from human erythrocytes.

Clonality

Monoclonal, clone number: 3B1

Isotype

Rabbit IgG

Form

Liquid

Preservative: 0.02% Sodium Azide

Constituents: PBS, pH 7.4

Storage instruction

store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

GAPDH作为内参蛋白，在细胞内恒定表达，具有甘油醛-3-磷酸脱氢酶和亚硝酸酶活性，因此分别在糖酵解和核功能中发挥作用。参与核事件，包括转录、RNA转运、DNA复制和凋亡。甘油醛-3-磷酸脱氢酶是糖酵解中的一种关键酶，通过将D-甘油醛3-磷酸酯（G3P）转化为3-磷酸-D-甘油酰基磷酸酯来催化途径的第一步。

描述

抗GAPDH兔单克隆抗体

识别种属

大鼠，小鼠，人类

已测试的应用实验

效价：1/2000-1/10000

尚未在其他应用中测试。

最佳稀释/浓度应由最终用户确定。

免疫原

来自人红细胞的全长GAPDH天然蛋白。

克隆性

单克隆，克隆号：3B1

抗体亚型

兔IgG

成分

液体

防腐剂：0.02%抑菌剂

缓冲体系：PBS，pH 7.4

存储说明

-20°C。避免重复冷冻/解冻循环。

All lanes : Anti-GAPDH rabbit monoclonal antibody at 1/5000 dilution

Lane 1 : HeLa cell lysate

Lane 2 : A549 cell lysate

Lane 3 : A431 cell lysate

Lane 4 : MCF7 cell lysate

Lane 5 : K562 cell lysate

Lane 6 : Jurkat cell lysate

Lane 7 : HL60 cell lysate

Lane 8 : SKN-SH cell lysate

Lane 9 : SKBR-3 cell lysate

Predicted band size : 36 kDa