Anti-Myc tag小鼠单克隆抗体

产品名称：Anti-Myc tag小鼠单克隆抗体

货号： AT0023

品牌：Engibody

简介：Myc tag标签抗体，亲和力高，特异性好，适用于检测细菌、酵母、昆虫细胞或哺乳动物细胞表达的包含Myc tag标签的融合蛋白，无论Myc tag标签处于融合蛋白的N端，C端还是中间，都可以

一管搞定全部实验（WB，IHC，ICC，IF及IP/CoIP）

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.

Description

Mouse monoclonal antibody to Myc-Tag - Epitope Tag Antibody

Reactivity

All Species Expected

Tested applications

WB    : 1/2000-1/10000.

IHC    : 1/200-1/5000.

ICC/IF    : 1/200-1/2000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

The antibody recognizes the Myc tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

Immunogen

a synthetic peptide corresponding to residues 410-419 of human c-Myc (EQKLISEEDL) conjugated to KLH

Clonality

Monoclonal, clone number: 1C9

Isotype

Mouse IgG1

Form

Liquid, 100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.

Storage instruction

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Database links

Sequence: EQKLISEEDL

Host

Mouse

表位标签可用于使用免疫印迹、免疫沉淀和免疫染色技术标记和检测蛋白质。由于其体积小，它们不太可能影响标记蛋白的生化特性。

Myc表位标签广泛用于检测重组蛋白在细菌、酵母、昆虫和哺乳动物细胞系统中的表达。

描述

抗Myc Tag抗体的小鼠单克隆抗体

反应性

预计所有物种

已测试的应用实验

WB:1/2000-1/10000。

IHC:1/200-1/5000。

ICC/IF:1/2000-1/2000。

IP/CoIP:1/100-1/500。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

抗体识别融合到转染细胞中靶蛋白的氨基或羧基末端的Myc标签。

免疫原

对应于与KLH偶联的人c-Myc（EQKLISEEDL）的残基410-419的合成肽

克隆性

单克隆，克隆号：1C9

同型对照

小鼠IgG1

类型

液体，100μg，1mg/mL

小鼠单克隆免疫球蛋白，磷酸盐缓冲盐水（PBS），pH 7.3（+/-0.1），50%甘油，0.1mg/mL BSA（IgG，无蛋白酶）作为稳定剂，0.02%防腐剂。

存储说明

-20°C下，避免冷冻/解冻循环。

序列：

EQKLISEEDL

抗体来源宿主

小鼠

Western Blot: Myc Epitope Tag Antibody - Analysis of Non-transfected (-) and Myc-tagged protein-transfected (+), 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Myc-tag antibody diluted by 1:10000.

Immunofluorescence: Myc Epitope Tag Antibody - Analysis of Myc tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Myc-recombinant protein stained by Myc tag antibody diluted at 1:2000. Blue: Hoechst 33342 staining.