Anti-HA tag小鼠单克隆抗体

产品名称：Anti-HA tag小鼠单克隆抗体

货号： AT0024

品牌：Engibody

简介：HA标签抗体，亲和力高，特异性好，适用于检测细菌、酵母、昆虫细胞或哺乳动物细胞表达的包含HA标签的融合蛋白，无论HA标签处于融合蛋白的N端，C端还是中间，都可以

The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors.

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Description

Mouse monoclonal antibody to HA tag - Epitope Tag Antibody

Reactivity

All Species Expected

Tested applications

WB    : 1/2000-1/10000.

IHC    : 1/200-1/5000.

ICC/IF    : 1/200-1/2000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

The antibody recognizes exogenously expressed proteins containing the HA epitope tag.

Immunogen

YPYDVPDYA (influenza hemagglutinin-HA-epitope) conjugated to KLH.

Clonality

Monoclonal, clone number: 6A1

Isotype

Mouse IgG2a

Form

Liquid, 100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.

Storage instruction

Aliquot and store at -20°C, Avoid freeze / thaw cycle.

Database links

Sequence: YPYDVPDYA (influenza hemagglutinin-HA-epitope).

Host

Mouse

HA标签源自流感血凝素蛋白的表位，该表位已广泛用作表达载体中的一般表位标签。

人流感血凝素（HA）是一种表面糖蛋白，对人类病毒的感染性是必需的。HA标签源自对应于氨基酸98-106的HA分子，已广泛用作表达载体中的一般表位标签。许多重组蛋白已被工程化以表达HA标签，这似乎不会干扰重组蛋白的生物活性或生物分布。这种标签有助于蛋白质的检测、分离和纯化。

描述

抗HA标签的小鼠单克隆抗体

反应性

所有物种

已测试的应用实验

WB:1/2000-1/10000。

IHC:1/200-1/5000。

ICC/IF:1/2000-1/2000。

IP/CoIP:1/100-1/500。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

抗体识别含有HA表位标签的外源表达蛋白。

免疫原

与KLH偶联的YPYDVPDYA（流感血凝素HA表位）。

克隆性

单克隆，克隆号：6A1

同型对照

小鼠IgG2a

类型

液体，100μg，1mg/mL

小鼠单克隆免疫球蛋白，磷酸盐缓冲盐水（PBS），pH 7.3（+/-0.1），50%甘油，0.1mg/mL BSA（IgG，无蛋白酶）作为稳定剂，0.02%防腐剂。

存储说明

-20°C下，避免冷冻/解冻循环。

序列：

YPYDVPDYA（流感血凝素HA表位）。

抗体来源宿主

小鼠

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged LGI-2, Internal Sequence HA-tagged CIGALT, and C-terminal HA-tagged FBLN. PVDF membrane was probed with 1:5000 of Anti-HA Antibody (Catalog # AT0024, Engidody) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # AT0098, Engidody). Specific bands were detected for HA-tagged proteins at approximately 60, 37, and 150 kDa (as indicated).

Immunocytochemistry/Immunofluorescence: HA Epitope Tag Antibody - Analysis of HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.

Red: HA-recombinant protein stained by HA tag antibody diluted at 1:1000.

Blue: Hoechst 33342 staining.

Immunoprecipitation and Western Blot: Anti-HA Epitope Tag Antibody - Analysis of 293T whole cell lysate/extract

A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells

B: Control with 1:5000 of mouse normal IgG

C: Immunoprecipitation of HA tagged protein by 1:200 of HA tag antibody, the immunoprecipitated HA tagged protein was detected by HA tag antibody diluted at 1:5000.