Anti-GST tag小鼠单克隆抗体

产品名称：Anti-GST tag小鼠单克隆抗体

货号：AT0027

品牌：Engibody

简介：GST标签抗体，一管搞定全部实验（WB，IHC，ICC，IF及IP/CoIP）

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade.

Description

Mouse monoclonal antibody to GST tag - Epitope Tag Antibody

Reactivity

All Species Expected

Tested applications

WB    : 1/2000-1/10000.

IHC    : 1/200-1/5000.

ICC/IF    : 1/200-1/2000.

IP/CoIP    : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects transfected GST fusion proteins.

Immunogen

Glutathione-S-Transferase (GST) from Schistosoma japonicum.

Clonality

Monoclonal, clone number: 2B8

Isotype

Mouse IgG2a

Form

Liquid,100 μg at 1mg/mL

Mouse monoclonal immunoglobulin in phosphate buffered saline (PBS), pH 7.3 (+/- 0.1), 50% glycerol with 0.1mg/mL BSA (IgG, protease free) as a stabilizer and 0.02% sodium azide as preservative.

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle

Database links

Sequence: GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.

Host

Mouse

GST是一种广泛使用的融合伴侣，因为它提供了易于检测的Tag和简单的纯化过程，对目标蛋白的生物功能几乎没有影响。在过去的十年中，已经为原核和真核系统开发了许多含有GST标签的载体。

描述

抗GST标签的小鼠单克隆抗体-表位标签抗体

反应性

能表达GST融合蛋白的所有物种

已测试的应用实验

WB:1/2000-1/10000。

IHC:1/200-1/5000。

ICC/IF:1/2000-1/2000。

IP/CoIP:1/100-1/500。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

该抗体检测转染的GST融合蛋白。

免疫原

日本血吸虫GST

克隆号

单克隆，克隆号：2B8

同型对照

小鼠IgG2a

类型

液体，100μg，1mg/mL

小鼠单克隆免疫球蛋白，磷酸盐缓冲盐水（PBS），pH 7.3（+/-0.1），50%甘油，0.1mg/mL BSA（IgG，无蛋白酶）作为稳定剂，0.02%防腐剂。

存储说明

-20°C下，避免冷冻/解冻循环

序列

GST是由日本血吸虫寄生蠕虫编码的26kDa蛋白，广泛用于GST质粒表达载体的pGEX家族，作为与外源蛋白的融合蛋白。

抗体来源宿主

小鼠

Western Blot: GST Epitope Tag Antibody - Analysis of extracted from transformed E. coli which expressing GST-tagged protein, using GST-tag antibody.

A: Non-induction E. coli lysate

B: Induction E. coli lysate; antibody diluted at 1:5000.

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number:AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Recombinant GST protein (20ng, Lane1) and 293 cellular lysates transfected with a GST expression vector (20μg, Lane2) were probed with anti-GST antibody (1:5000). Arrow indicates GST(~26kDa) and GST-fusion protein(~29kDa).

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number: AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)