EGFP抗体

产品名称：Anti-EGFP小鼠单克隆抗体

货号： AT0041

品牌：Engibody

简介：EGFP标签抗体，特异性识别EGFP标签，一管搞定全部实验（WB，IHC，ICC，IF及IP/CoIP）

免疫原

增强型绿色荧光蛋白（EGFP）重组蛋白。

克隆号

7K15

同型对照

小鼠IgG1

类型

液体

小鼠单克隆抗体在10 mM PBS中，pH 7.4，50%甘油，1 mg/mL（0.1%w/v）BSA（不含IgG，不含蛋白酶）作为稳定剂，0.02%（w/v）防腐剂。

存储说明

长期存放于-20°C。避免重复冷冻/解冻循环。

Description

anti-EGFP-Tag mouse monoclonal antibody

Reactivity

Any organism expressing EGFP.

Tested applications

WB : 1/1000-1/10000.

IHC : 1/200-1/5000.

ICC/IF : 1/200-1/2000.

IP : 1/100-1/500.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins.

Please note that the EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

The antibody recognizes the EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.

Western blot - anti-EGFP-Tag mouse mAb (AT0041)

All lanes:

Lane: HEK293T cell lysate transfected with EGFP-Tagged protein, anti-EGFP-Tag mouse monoclonal antibody at 1/5000 dilution

Lysates/proteins at 25 µg per lane.

Recommended secondary antibody: Goat anti mouse IgG-HRP (Engibody, Catalog number: AT0098)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)