Anti-Strep-tag II小鼠单克隆抗体

产品名称：Anti-Strep-tag II小鼠单克隆抗体

货号： AT0817

品牌：Engibody

简介：Strep-tag II标签抗体

Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag (AWRHPQFGG) was a nine amino acid peptide with high specificity and affinity towards streptavidin which allows the simple purification of protein by use of affinity columns, but required addition to only the C-terminus of recombinant proteins. To also allow a Strep-tag to be placed at the N-terminus of recombinant proteins, it was re-engineered and re-named Strep-tag II (WSHPQFEK).

Description

Anti-Strep-tag II Mouse monoclonal antibody

Tested applications

WB    : 1/1000-1/5000.

Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody can recognize C-terminal, internal, and N-terminal Strep-tag II fusion proteins.

Immunogen

A synthetic peptide WSHPQFEK coupled to KLH.

Clonality

7D2

Isotype

Mouse IgG1

Form

Liquid, 1mg/ml

Preservative: 0.01% Sodium Azide

Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Storage instruction

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Host

Mouse

链霉亲和素是从阿维迪尼链霉菌中纯化的四聚体蛋白。由于其biotin的强亲和力，它在许多分子生物学协议中有广泛的应用。原始Strep标签（AWRHPQFGG）是一种九氨基酸肽，对链霉亲和素具有高度特异性和亲和力，允许通过亲和柱简单纯化蛋白质，但只需要添加到重组蛋白的C末端。为了将Strep标签放置在重组蛋白的N末端，对其进行了重组，并将其重新命名为Strep标签II（WSHPQFEK）。

描述

抗Strep-tag II小鼠单克隆抗体

已测试的应用实验

WB:1/1000-1/5000。

尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。

特异性

该抗体可识别C端、内部和N端Strep-tag II融合蛋白。

免疫原

一种与KLH偶联的合成肽WSHPQFEK。

克隆性

7D2

同型对照

小鼠IgG1

类型

液体，1mg/ml

防腐剂：0.01%

成分：0.15M氯化钠，0.02M磷酸钾，pH 7.2

存储说明

在-20°C储存。避免重复冷冻/解冻循环。

抗体来源宿主

小鼠

All lanes : Anti-Strep-tag II antibody at 1/5000 dilution

Lane 1 : 293T whole cell lysate

Lane 2 : whole cell lysate of strep-tagged II protein expressing 293T cells

Lysates/proteins at 30 µg per lane.

12 % SDS-PAGE