
产品名称:Anti-MBP小鼠单克隆抗体
货号: AT0030
品牌:Engibody
简介:MBP标签抗体
Maltose binding protein (MBP) is a useful affinity tag that can increase the expression level and solubility of the MBP tagged protein. It promotes proper folding of the fusion protein, and it can be also used to prevent an insoluble form (inclusion bodies). MBP-tag antibody is suitable for detecting fusion proteins that contain a MBP-tag.
Description
Mouse monoclonal antibody to MBP-Tag [7B10] - Epitope Tag Antibody
Reactivity
Species independent
Tested applications
WB : 1/1000-1/5000.
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Specificity
This antibody detects recombinant proteins fused to maltose binding protein (MBP).
Immunogen
Recombinant full-length MBP protein.
Clonality
7B10
Isotype
Mouse IgG1
Form
Liquid, 100 μg at 1mg/ml
Mouse monoclonal antibody in 10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 1 mg/mL (0.1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.02% (w/v) sodium azide as preservative.
Storage instruction
Store at -20°C
麦芽糖结合蛋白(MBP)是一种有用的亲和标签,可以提高MBP标记蛋白的表达水平和溶解度。它促进融合蛋白的正确折叠,也可用于防止不溶性形式(包涵体)。MBP标签抗体适合于检测包含MBP标签的融合蛋白。
描述
抗MBP-Tag[7B10]-表位标签抗体的小鼠单克隆抗体
反应性
与物种无关
已测试的应用实验
WB:1/1000-1/5000。
尚未在其他应用中测试。最佳稀释/浓度应由最终用户确定。
特异性
该抗体检测与麦芽糖结合蛋白(MBP)融合的重组蛋白。
免疫原
重组全长MBP蛋白。
克隆性
7B10
同型对照
小鼠IgG1
类型
液体,100μg,1mg/ml
小鼠单克隆抗体在10 mM磷酸盐缓冲盐水(PBS)中,pH 7.4,50%甘油,1 mg/mL(0.1%w/v)BSA(不含IgG,不含蛋白酶)作为稳定剂,0.02%(w/v)防腐剂。
存储说明
在-20°C储存
Western blot analysis of MBP-tag fusion protein with anti MBP-tag mouse mAb [7B10] diluted at 1: 5000.