Anti-GFP兔单抗

产品名称：Anti-GFP兔单抗

货号： AT1598

品牌：Engibody

简介：GFP兔单抗，一管搞定全部实验（WB，IHC，ICC，IF及IP/CoIP）

Green fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

Tested applications

WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

This antibody detects GFP, EGFP -tagged proteins exogenously expressed in cells or E. coli. This antibody does not detect RFP-tagged proteins.

Please note that the GFP, EGFP tags add approximately 27 kDa to the molecular weight of the fusion protein.

The antibody recognizes the GFP, EGFP tag, which is fused to either the amino-terminus or carboxy-terminus of the target protein.

Immunogen

a Green Fluorescent Protein (GFP) recombinant protein.

Clonality

Monoclonal, clone number: 7D5

Isotype

Rabbit IgG

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

Store at -20°C

绿色荧光蛋白（GFP）是一种27kDa的蛋白质，来源于维多利亚水母，发出绿光。GFP已被广泛用作基因表达的报告，使研究人员能够在活细胞内可视化和定位GFP标记蛋白，而无需化学染色。YFP由于T203Y突变而与GFP不同；针对全长GFP产生的抗体也应检测YFP和其他变体。

已测试的应用实验

WB:1/1000-1/5000。

IHC:1/200-1/500。

ICC/IF:1/100-1/500。

IP：1/50-1/200。

最佳稀释/浓度应由最终用户确定。

特异性

这种抗体检测细胞或大肠杆菌中外源表达的GFP、EGFP标记蛋白。该抗体不能检测RFP标记的蛋白质。

请注意，GFP、EGFP标签使融合蛋白的分子量增加了约27kDa。

抗体识别GFP，即EGFP标签，融合到目标蛋白的氨基末端或羧基末端。

免疫原

绿色荧光蛋白（GFP）重组蛋白。

克隆性

单克隆，克隆号：7D5

同型对照

兔IgG

类型

液体，100μg，1mg/ml

存储说明

在-20°C下储存

Western Blot: GFP Antibody - Analysis of extracts from 293A cells, unstransfected (lane 1) and transfected (lane 2) with GFP expression vector, using GFP antibody at 1:1000.