Anti-Myc兔单抗

产品名称：Anti-Myc兔单抗

货号： AT0045

品牌：Engibody

简介：myc兔单克隆抗体，Myc标签融合蛋白的检测（WB,IHC,IF,IP,CoIP）

Description

Rabbit monoclonal antibody to myc tag

Reactivity

All species

Tested applications

WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

EQKLISEEDL(C-MYC) conjugated with KLH.

Clonality

Monoclonal, clone number: 5D12

Isotype

Rabbit IgG

Form

Liquid, 100 ul, at 1mg/ml with PBS, pH 7.4, containing 0.02% sodium azide

Storage instruction

Store at -20°C. Avoid repeated freezing and thawing cycles.

描述

抗myc标签兔单克隆抗体

反应性

所有表达Myc融合蛋白的物种

已测试的应用实验

WB:1/1000-1/5000。

IHC:1/200-1/500。

ICC/IF:1/100-1/500。

IP：1/50-1/200。

最佳稀释/浓度应由最终用户确定。

免疫原

EQKLISEEDL（C-MYC）与KLH偶联。

克隆性

单克隆，克隆号：5D12

同型对照

兔IgG

类型

液体，100 ul，含1mg/ml PBS，pH 7.4，含0.02%抑菌剂

存储说明

储存在-20°C。避免重复的冷冻和解冻循环。

Western blot

Sample Human Cell lysate - whole cell (293T)

Loading amount 25 µg

Treatment Cells transfected with pCMV-Myc

Gel Running Conditions Reduced Denaturing

Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s)

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)