Anti-DYKDDDDK (FLAG) 小鼠单克隆抗体 - ChIP Grade

产品名称：Anti-DYKDDDDK (FLAG) 小鼠单克隆抗体 - ChIP Grade

货号： AT2108

品牌：Engibody

简介：flag抗体ChIP级，DYKDDDDK抗体ChIP级别，用于ChIP实验，兼做WB，IHC，IF及IP/CoIP

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.

The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

Description

Mouse monoclonal antibody to DYKDDDDK (FLAG®) tag - ChIP Grade (Binds to same epitope as Sigma's Anti-FLAG M2 Antibody)

Tested applications

WB      :1/2000-1/10000

IP/CoIP:1/50-1/500

ChIP    : 1/20-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

Synthetic peptide: DYKDDDDK conjugated to KLH

Clonality

Monoclonal, clone number: 4D1

Isotype

Mouse IgG1

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

Store at -20°C

表位标签可用于使用免疫印迹、免疫沉淀和免疫染色技术标记和检测蛋白质。由于它们的体积小，它们不太可能影响标记蛋白的生化特性。

DYKDDDDK肽已广泛用作表达载体中的一般表位标签。该肽可以作为氨基末端或羧基末端融合物与感兴趣的蛋白质一起表达和检测。

描述

抗DYKDDDDK（FLAG®）标签的小鼠单克隆抗体-ChIP级（与Sigma的抗FLAG M2抗体结合相同表位）

经过测试的应用实验

效价

WB：1/2000-1/10000

IP/CoIP：1/50-1/500

ChIP：1/20-1/200。

最佳稀释度/浓度应由最终用户确定。

免疫原

合成肽：与KLH偶联的DYKDDDDK

克隆性

单克隆，克隆号：4D1

同型对照

小鼠IgG1

类型

液体，100μg，1mg/ml

存储说明

储存温度-20°C

Western Blot: DYKDDDDK Epitope Tag Antibody (AT2108) - Analysis of 40 ug whole cell extracts from HeLa cells transfected with either DYKDDDDK-tagged MBD1 (lanes 1 and 3) or DYKDDDDK-tagged MBD2 (lane 2 and 4). The WB was performed with the DYKDDDDK Epitope Tag Antibody (AT2108) diluted 1:5000 in TBS-Tween containing 5% skimmed milk (lane 3 and 4) or with an MBD1 antibody (lane 1) or an MBD2 antibody (lane 2).

Chromatin Immunoprecipitation: DYKDDDDK Epitope Tag Antibody - ChIP results obtained with the antibody against the DYKDDDDK tag. ChIP assays were performed using Plasmodium falciparium cells stably expressing DYKDDDDK-H2A (blue bars) and wild type Plasmodium falciparium cells (yellow bars). Primer pairs were used for active genes, inactive genes, and variable genes. Sheared chromatin from 1x10e6 cells and 10 ug of antibody against DYKDDDDK were used per ChIP experiment.