Anti-GFP兔多抗 - ChIP Grade

产品名称：Anti-GFP兔多抗 - ChIP Grade

货号：AT0044

品牌：Engibody

简介：GFP标签融合蛋白的ChIP检测（兼具WB,IHC,IF,IP,CoIP）

Green fluorescence protein (GFP) is a 27 kDa protein derived from jellyfish Aequorea victoria. It emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become a very useful tool as a fusion protein to report gene expression, trace cell lineage and define subcellular protein localizations. YFP differs from GFP due to a mutation at T203Y.

Description

Anti-GFP Rabbit pAb, ChIP Grade

Reactivity

Anti-GFP Antibody detects GFP-tagged proteins within cell lysates

Tested applications

WB (1:1000-5000), IP (1:100-300), IF (1:150-500),

Flow Cyt, ChIP, ChIP/Chip, Electron Microscopy, IHC-P, IHC-Fr

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

Highly purified recombinant full length protein made in Escherichia coli. The antibody is directed against the entire GFP molecule.

Clonality

Polyclonal

Isotype

Rabbit IgG

Form

Liquid

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

绿色荧光蛋白（GFP）是一种27kDa的蛋白质，来源于海蜇Aequorea victoria。当被蓝光（波长395nm处的激发峰）激发时，它发射绿光（波长509nm处的发射峰）。GFP作为一种融合蛋白已成为一种非常有用的工具，用于报告基因表达、追踪细胞谱系和确定亚细胞蛋白定位。由于T203Y的突变，YFP与GFP不同。

描述

抗GFP兔pAb，ChIP级

反应性

细胞或细菌裂解物中的GFP标记蛋白

经过测试的应用实验

WB（1:1000-5000），IP（1:100-300），IF（1:150-500），

流式细胞术、ChIP、ChIP/芯片、IHC-P、IHC-Fr

最佳稀释度/浓度应由最终用户确定。

免疫原

高度纯化的重组GFP全长蛋白，由大肠杆菌制成。抗体针对整个GFP分子。

克隆性

多克隆的

同型对照

兔IgG

类型

液体

存储说明

储存于-20°C，避免冷冻/解冻循环。

Anti-GFP antibody (AT0044) at 1/2000 dilution + Lysate prepared from rabbit reticulocytes at 30 µg

Developed using the ECL technique

Performed under reducing conditions.

Observed band size: 27 kDa

Exposure time: 5 seconds

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

AT0044 staining dog hearts (Adv-GFP injection) tissue sections by IHC-P.  Sections were PFA fixed and subjected to heat mediated antigen retrieval in citric acid (Ph6.0, 0.05% Tween20) prior to blocking with 10% serum for 30 mins at 37°C.  The primary antibody was diluted 1/500 in PBS and incubated with the sample for 1 hour at 25°C.

Immunofluorescence images showing similar localization of Yes-GFP (first 10 aa's of Yes PTK fused to the N-terminus of GFP) to full length Yes PTK.

A: Distribution of Yes detected using mouse anti-Yes Ab followed by Texas Red-conjugated anti-mouse Ab.

B: Chimeric GFP's detected using rabbit anti-GFP rabbit Ab (CAT: AT0044) followed by FITC-conjugated anti-rabbit pAb.