Anti-HA兔单抗 - ChIP Grade

产品名称：Anti-HA兔单抗 - ChIP Grade

货号：AT1747

品牌：Engibody

简介：HA标签融合蛋白的ChIP检测（兼做WB,IHC,IF,IP,CoIP）

Specificity

HA Antibody Recognizes HA-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.

Reactivity

All species

Tested applications

WB : 1/1000-1/5000.

IHC : 1/200-1/500.

ICC/IF : 1/100-1/500.

IP : 1/50-1/200.

ChIP : 1/50-1/100.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

Synthetic peptide: YPYDVPDYA conjugated to KLH

Clonality

Monoclonal, clone number:1E6

Isotype

Rabbit IgG

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

store at -20°C

特异性

HA抗体识别细胞中过度表达的HA标记蛋白，包括转染哺乳动物细胞中靶向蛋白的氨基和羧基末端。

反应性

所有表达HA融合蛋白的物种

经过测试的应用实验

WB：1/1000-1/5000。

IHC:1/200-1/500。

ICC/IF:1/100-1/500。

IP：1/50-1/200。

ChIP：1/50-1/100。

最佳稀释度/浓度应由最终用户确定。

免疫原

合成肽：与KLH偶联的YPYDVPDYA

克隆性

单克隆，克隆号：1E6

同型对照

兔IgG

类型

液体，100μg，1mg/ml

存储说明

储存温度-20°C

All lanes: Anti-HA tag antibody- ChIP grade- (AT1747) at 1/5000 dilution

Lane 1: HEK293 human embryonic kidney cell line transfected with N-terminal HA-tagged MICA

Lane 2: HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged TRANCE

Lane 3: HEK293 human embryonic kidney cell line transfected with C-terminal HA-tagged LRRN5

Recommended secondary antibody: Goat anti rabbit IgG-HRP (Engibody, Catalog number:AT0097)

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)

Immunocytochemistry:

in immersion fixed NS0 mouse myeloma cell line transfected with HA-tagged WISP-2 using Rabbit Anti-HA Monoclonal Antibody – ChIP Grade

Recommended secondary antibody: Goat Anti-Rabbit IgG(H+L) (IFluor™ 594) (Engibody, Catalog number:AT1553)

Xenopus laevis oocytes were injected with mRNA for HA-tagged human BORIS. Chromatin was prepared according to the ChIP protocol. Oocytes were fixed with formaldehyde for 10min.

The ChIP was performed with 25µg of chromatin, 20µl of Protein A/G agarose beads, and 5µg of anti-HA antibody, light blue) or, 5µg of anti-Boris antibody, dark blue). A non-specific antibody was used as a control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach).