Anti-Myc兔单抗 - ChIP Grade

产品名称：Anti-Myc标签兔单抗 - ChIP Grade

货号： AT1746

品牌：Engibody

简介：Myc标签融合蛋白的ChIP检测（兼做WB,IHC,IF,IP,CoIP）

Reactivity

All species

Tested applications

IP/CoIP:1/50-1/500

ChIP    : 1/20-1/200.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

c-Myc Antibody Recognizes c-Myc-tagged proteins overexpressed in cells, including both amino- and carboxy-termini of targeted proteins in transfected mammalian cells.

Immunogen

Synthetic peptide: EQKLISEEDL, corresponding to amino acids 410-419 of Human c-Myc.

Clonality

Monoclonal, clone number:3E12

Isotype

Rabbit IgG

Form

Liquid, 100 μg at 1mg/ml

Storage instruction

Store at -20°C

反应性

所有表达myc融合蛋白的物种

经过测试的应用实验

IP/CoIP：1/50-1/500

ChIP：1/20-1/200。

最佳稀释度/浓度应由最终用户确定。

特异性

c-Myc抗体识别细胞中过度表达的c-Myc标记蛋白，包括转染哺乳动物细胞中靶向蛋白的氨基和羧基末端。

免疫原

合成肽：EQKLISEEDL，对应于人类c-Myc的氨基酸410-419。

克隆性

单克隆，克隆号：3E12

同型对照

兔IgG

类型

液体，100μg，1mg/ml

存储说明

在-20°C下储存

A stably transfected 293T human cell line harbouring the GAL4 upstream activation sequence was transiently transfected with a Myc or His - tagged GAL4 DNA Binding Domain construct. 48 hours post transfection Chromatin was prepared according to the ChIP protocol. The ChIP was performed with 25 ug chromatin, 5ug of antibody and 20 ul of Protein A/G beads. A non-specific antibody was used as the negative control. The immunoprecipitated DNA was quantified by real time PCR (SYBR Green approach).