Anti-GFP小鼠单抗标记HRP

产品名称：Anti-GFP小鼠单抗标记HRP

货号： AT0055

品牌：Engibody

简介：HRP标记GFP抗体，用于WB实验，无需二抗

Description

Mouse monoclonal antibody to GFP[7D10] Conjugated Peroxidase (HRP)

Tested applications

WB : 1/5000-1/10000.

Optimal dilutions/concentrations should be determined by the end user.

Specificity

The antibody recognizes the GFP, EGFP fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

There is no need using secondary antibody.

Immunogen

a Green Fluorescent Protein (GFP) recombinant protein.

Clonality

7D10

Isotype

Mouse IgG2a

Form

10 mM phosphate buffered saline (PBS), pH 7.4, 50% glycerol with 10 mg/mL (1% w/v) BSA (IgG free, protease free) as a stabilizer and 0.01% (w/v) thimerosal as preservative.

Storage instruction

Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.

描述

偶联过氧化物酶（HRP）的抗GFP[7D10]小鼠单克隆抗体

经过测试的应用实验

WB:1/5000-1/10000。

不需要使用二抗。

最佳稀释度/浓度应由最终用户确定。

特异性

抗体识别与转染细胞中靶蛋白的氨基或羧基末端融合的GFP、EGFP。

免疫原

绿色荧光蛋白（GFP）重组蛋白。

克隆号

7D10

同型对照

小鼠IgG2a

类型

10mM磷酸盐缓冲盐水（PBS），pH 7.4，50%甘油，10mg/mL（1%w/v）BSA（不含IgG，不含蛋白酶）作为稳定剂，0.01%（w/v）硫柳汞作为防腐剂。

存储说明

储存于-20°C，避免冷冻/解冻循环。不要分装抗体。

Western Blot: GFP Antibody-HRP- Analysis of extracts from Hela cells,  transfected with GFP tagged protein expression vector, using GFP antibody-HRP (AT0055) at 1:5000.

Recommended ECL: ECL Pico-Detect™ Western Chemiluminescent HRP Substrate (Engibody, Catalog number: IF6747)