Anti-GST小鼠单抗标记HRP

产品名称：Anti-GST小鼠单抗标记HRP

货号： AT0053

品牌：Engibody

简介：Anti-GST小鼠单抗标记HRP，WB实验无需二抗

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade.

Description

HRP conjugated Mouse monoclonal antibody to GST

Reactivity

GST mouse mAb (HRP Conjugated) detects transfected GST fusion proteins expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Tested applications

WB : 1/500-1/5000.

Optimal dilutions/concentrations should be determined by the end user.

Immunogen

GST fusion protein

Isotype

Mouse IgG1

Form

Liquid

Preservative: 15mM Sodium Azide

Constituents: PBS, pH 7.4

Storage instruction

Store at -20°C. Avoid repeated freeze / thaw cycles.

表位标签可用于使用免疫印迹、免疫沉淀和免疫染色技术标记和检测蛋白质。由于它们的体积小，它们不太可能影响标记蛋白的生化特性。

GST是一种广泛使用的融合伴侣，因为它提供了一种易于检测的标签和一种简单的纯化过程，对目标蛋白的生物学功能几乎没有影响。在过去的十年中，已经为原核和真核系统开发了许多含有GST-Tag的载体。

描述

HRP标记的抗GST小鼠单克隆抗体

反应性

GST小鼠单克隆抗体（HRP指标）检测在大肠杆菌、酵母、昆虫细胞、哺乳动物和体外转录/翻译系统中表达的转染GST融合蛋白。

经过测试的应用实验

WB：1/500-1/5000。

无需二抗

最佳稀释度/浓度应由最终用户确定。

免疫原

GST融合蛋白

同型对照

小鼠IgG1

类型

液体

防腐剂：15mM

成分：PBS，pH 7.4

存储说明

在-20°C下保存。避免重复冷冻/解冻循环。

Western blot analysis of extracts from COS-7 cells, transfected with a construct overexpressing GST-tagged protein, using GST mouse mAb (HRP Conjugated) at 1:1000 dilution.