Anti-V5小鼠单抗标记HRP

产品名称：Anti-V5小鼠单抗标记HRP

货号： AT0058

品牌：Engibody

简介：Anti-V5小鼠单抗标记HRP，用于V5蛋白的WB检测，无需二抗

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. The V5 tag can be used to detect expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.

Description

HRP conjugated Mouse monoclonal antibody to V5

Reactivity

V5-Tag mouse mAb (HRP Conjugated) detects transfected V5 fusion proteins expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Tested applications

WB : 1/500-1/1000.

Optimal dilutions/concentrations should be determined by the end user.

• Immunogen

• Monoclonal antibody is produced by immunizing animals with a synthetic V5-tag peptide.

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• Isotype

• Mouse IgG1

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• Form

• Liquid
  Preservative: 15mM Sodium Azide
  Constituents: PBS, pH 7.4

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• Storage instruction

• Store at -20°C. Avoid repeated freeze / thaw cycles.

表位标签可用于使用免疫印迹、免疫沉淀和免疫染色技术标记和检测蛋白质。由于它们的体积小，它们不太可能影响标记蛋白的生化特性。

V5标签是一种14个氨基酸的肽，来源于副粘病毒家族成员猿猴病毒5（SV5）的P和V蛋白上的小表位（Pk）。V5标签可用于检测重组蛋白在细菌、酵母、昆虫和哺乳动物细胞系统中的表达。

产品描述

HRP偶联的小鼠V5单克隆抗体

反应性

V5标记小鼠mAb（HRP标记）检测在大肠杆菌、酵母、哺乳动物和体外转录/翻译系统中表达的转染的V5融合蛋白。

经过测试的应用实验

WB：1/500-1/1000。

无需二抗

最佳稀释度/浓度应由最终用户确定。

免疫原

抗体是通过用合成的V5标签肽免疫动物而产生的。

同型对照

小鼠IgG1

类型

液体

防腐剂：15mM

成分：PBS，pH 7.4

存储说明

在-20°C下保存。避免重复冷冻/解冻循环

Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with V5-tag fusion protein (+), using V5-Tag mouse mAb and β-Actin mouse mAb(AT0001)