Goat Anti-Mouse IgG(H+L) (iFluor 488)

简要描述：Goat Anti-Mouse IgG(H+L) (iFluor 488) , iFluor 488直标荧光二抗

产品型号：AT1547
厂商性质：代理商
更新时间：2023-03-23
访问量：149

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详情介绍

产品名称：Goat Anti-Mouse IgG(H+L) (iFluor 488)

货号： AT1547

品牌：Engibody

关键词： iFluor 488标记抗小鼠二抗,（等同于Alexa Fluor 488标记抗小鼠二抗, DyLight 488标记抗小鼠二抗）

By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and with light chains common to other Mouse immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

iFluor™ 488, Alternative to Alexa Fluor® 488 and DyLight® 488

Description

Goat-anti-mouse IgG(H+L)(iFluor™ 488)

Reactivity

mouse

Tested applications

ICC/IF: 1/100 - 1/2000,

Flow Cyt: The amount of the reagent is suggested to be used ≤ 1µg/106cells.

Clonality

Polyclonal

Form

Liquid

Storage instruction

Store at -20°C.and protected from prolonged exposure to light

Conjugation

iFluor™ 488. Ex: 493nm, Em: 518nm

通过免疫电泳和ELISA，该抗体与小鼠IgG和其他小鼠免疫球蛋白共有的轻链特异性反应。未检测到针对非免疫球蛋白血清蛋白的抗体。

iFluor™ 488，为Alexa Fluor®488和DyLight®488的替代品

描述

山羊抗小鼠IgG（H+L）（iFluor™ 488)

反应性

小鼠

经过测试的应用实验

ICC/IF:1/100-1/2000，

流式细胞术：建议使用的试剂量≤ 1µg/106个细胞。

克隆性

多克隆的

类型

液体

存储说明

储存于-20°C。并防止长时间暴露在阳光下

偶联荧光染料

iFluor™ 488. Ex:493nm，Em:518nm

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin mouse monoclonal antibody(AT0007)

staining alpha Tubulin in MEF1 cells. The cells were fixed with 4% formaldehyde (10 minutes), permeabilized in 0.1% PBS-Triton X-100 for 5 minutes then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1 hour. The cells were then incubated with AT0007 at 1/5000 dilution (shown in green) overnight at +4°C, then incubated with Goat Anti-Mouse IgG(H+L) (iFluor 488) (AT1547). Nuclear DNA was labeled in blue with DAPI.

This product gave a positive signal in 100% methanol (10 minutes) fixed MEF1 cells under the same testing conditions.

Image was taken with a Confocal microscope (Leica micro-systems, TCS SP8).

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