Goat Anti-Rabbit IgG(H+L) (iFluor 488)

产品名称：Goat Anti-Rabbit IgG(H+L) (iFluor 488)

货号：AT1546

品牌：Engibody

关键词：iFluor 488标记抗兔二抗,（等同于Alexa Fluor 488标记抗兔二抗, DyLight 488标记抗兔二抗） 

By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.

iFluor™ 488, Alternative to Alexa Fluor® 488 and DyLight® 488

Description

Goat-anti-rabbit IgG(H+L)(iFluor™ 488)

Reactivity

Rabbit

Tested applications

ICC/IF :1/100 - 1/2000.

Optimal dilutions/concentrations should be determined by the end user.

Clonality

Polyclonal

Form

Liquid

Storage instruction

Store at -20°C.and protected from prolonged exposure to light.

Conjugation

iFluor™ 488. Ex: 493nm, Em: 518nm

通过免疫电泳和ELISA，该抗体与兔IgG和其他兔免疫球蛋白共有的轻链特异性反应。未检测到针对非免疫球蛋白血清蛋白的抗体。检测到对牛、鸡、山羊、马、人、小鼠、猪和大鼠IgG的交叉反应性小于0.1%。

iFluor™ 488，为Alexa Fluor®488和DyLight®488的替代品

描述

山羊抗兔IgG（H+L）（iFluor™ 488)

反应性

兔子

经过测试的应用实验

ICC/IF：1/100-1/2000。

最佳稀释度/浓度应由最终用户确定。

克隆性

多克隆的

类型

液体

存储说明

储存于-20°C。并防止长时间暴露在光下。

偶联标记物（荧光染料）

iFluor™ 488    Ex:493nm，Em:518nm

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG(H+L) (iFluor™ 488)

ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (AT0011, Anti-beta Tubulin Rabbit mAb, 1:5000) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG(H+L) (iFluor™ 488) (AT1546, used at 1:2000 for 1h). DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.