Protein G抗体纯化填料

产品名称：Protein G agarose Resin 6FF for Antibody Purification， Protein G抗体纯化填料

货号： P2709

品牌：Engibody

简介：Protein G抗体纯化填料，用于小鼠和大鼠的单克隆抗体及多克隆抗体的纯化。

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. P2709 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100µl to 0.5ml. Up to 500µg of antibody can be purified in each run.

The method involves capturing the antibody on the Protein G resin. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein G, unwanted substances can be removed by simply washing the resin. Up to 500ug of antibody can be purified in each run.

Description

Protein G Resin for Antibody Purification

Tested applications

Antibody Purification

Form

50% slurry

Storage instruction

Store re-equilibrated Protein G Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.

Beads Diameter

90 μm

Binding Capacity

>30 mg human IgG/mL settled resin

Ligand

Recombinant protein G produced in E.coli

Matrix

Highly cross-linked 6% agarose beads

Maximum pressure

0.3 MPa (3 bar)

Chemical stability

All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

Flow rate

Gravity flow, 100 - 300 cm/hour

pH stability

Long term: pH3 - 9;

Short term: pH2 - 10

Storage buffer

1x PBS containing 20% ethanol

市售抗体通常含有干扰酶或荧光团标记反应的物质（例如BSA、三嗪、叠氮化物）。P2709快速去除这些污染物。它还可以用于从诸如腹水或免疫血清的粗样品中纯化抗体。待纯化或净化的抗体的理想体积为100µl至0.5ml。每次运行最多可纯化500µg抗体。

该方法包括在蛋白G树脂上捕获抗体。蛋白质G对来自各种物种的IgG分子的Fc区具有高亲和力。一旦抗体与G蛋白结合，可以通过简单地清洗树脂来去除不需要的物质。每次运行最多可纯化500ug抗体。

描述

抗体纯化用G蛋白偶联beads

经过测试的应用实验

抗体纯化

类型

50%slurry

存储说明

将重新平衡的G蛋白树脂储存在2°C至8°C含20%乙醇的结合/洗涤缓冲液中。不要冻结。

beads直径

90微米

结合能力

>30 mg人IgG/mL纯beads

配体

大肠杆菌中产生的重组蛋白G

基质

高交联6%琼脂糖珠

最大压力

0.3 MPa

化学稳定性

所有常用的水性缓冲液，包括6M盐酸胍和8M尿素。

流速

重力流，100-300 cm/小时

pH稳定性

Long term: pH3 - 9;

Short term: pH2 - 10

存储缓冲液

1x PBS，含20%乙醇

Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.