耐碱Protein A抗体纯化填料

产品名称：Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification， 耐碱Protein A抗体纯化填料

货号：P5555

品牌：Engibody

关键词：

耐碱Protein A Resin,

耐碱Protein A抗体纯化填料,

耐碱Protein A Sepharose

适用于人源单克隆抗体以及治疗性Fc重组蛋白的纯化

rProtein A Agarose 6 Fast Flow is a proprietary affinity chromatography medium designed for easy, one-step purification of immunoglobulins, notably monoclonal antibodies, from samples of larger volumes.

Alkali-tolerant protein A is engineered by gene recombination technology on the base of native protein A. Our Alkali-Tolerant Protein A Agarose 4FF retains ≥95% capacity after 200 times repeated clean-in-place (CIP) cycles with 0.1M NaOH, and approximately 80% of the initial capacity with 0.5M NaOH after 100 times repeated CIP cycles. Alkali-Tolerant Protein A is immobilized onto the cross-linked 6% agarose resin with an orientation that is optimal for immunoglobulin binding. The level of leakage of the ligand during elution is very low.

Description

Alkali-Tolerant Protein A agarose Resin 6FF for Antibody Purification

Tested applications

Antibody Purification

Form

50% Slurry

Storage instruction

Store at 2 - 8°C. Do not Freeze

Beads Diameter

an average of ~ 90 μm

Binding Capacity

>40 mg human IgG/mL settled resin

Ligand

Recombinant alkali-tolerant protein A produced in E.coli

Matrix

Highly cross-linked 6% agarose beads

Maximum pressure

0.3 MPa (3 bar)

Chemical stability

All commonly used aqueous buffers and reagents in the process of antibody purification, including 6 M guanidine-HCl and 8 M urea.

Flow rate

Gravity flow and medium pressure, 30 - 300 cm/hour

Storage buffer

1x PBS containing 20% ethanol

Cleaning-in-place

0.1 M ~ 0.5 M NaOH

rProtein A Agarose 6 Fast Flow是一种专有的亲和层析介质，设计用于从较大体积的样品中简单、一步纯化免疫球蛋白，特别是药物单克隆抗体。

耐碱蛋白A是在天然蛋白A的基础上通过基因重组技术改造的。我们的耐碱蛋白A-琼脂糖4FF保留了≥用0.1M NaOH进行200次重复原位清洗（CIP）循环后的容量为95%，用0.5M NaOH进行100次重复CIP循环后的初始容量约为80%。耐碱蛋白A以适合免疫球蛋白结合的取向固定在交联的6%琼脂糖树脂上。洗脱期间配体的泄漏水平非常低。

产品描述

用于抗体纯化的耐碱蛋白A琼脂糖树脂6FF

经过测试的应用实验

抗体纯化

类型

50%悬液slurry

存储说明

储存温度为2-8°C。请勿冻结

beads直径

平均约90μm

结合能力

>40 mg人IgG/mL沉淀树脂

配体

大肠杆菌中产生的重组耐碱蛋白A

基质

高交联6%琼脂糖珠

最大压力

0.3 MPa（3bar）

化学稳定性

抗体纯化过程中常用的所有水性缓冲液和试剂，包括6M盐酸胍和8M尿素。

流速

重力流和中压，30-300 cm/小时

存储缓冲液

1x PBS，含20%乙醇

在位清洗

0.1 M～0.5 M氢氧化钠