Protein L抗体轻链纯化填料

产品名称：Protein L agarose Resin 6FF for Antibody Purification， Protein L抗体轻链纯化填料

货号： P2710

品牌：Engibody

简介：

Protein L Resin,

抗体轻链纯化填料，

Protein L Sepharose

Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. P2710 quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100µl to 0.5ml. Up to 500µg of antibody can be purified in each run.

The method involves capturing the antibody on the Protein L resin. Protein L has a high affinity for the Fc regions of IgG molecules from a variety of species. Once the antibody has bound to the Protein L, unwanted substances can be removed by simply washing the resin. Up to 500ug of antibody can be purified in each run.

Description

Protein L Agarose beads for Antibody Purification

Tested applications

Antibody Purification

Form

10 ml 50% slurry

Storage instruction

Store Protein L Resin in Binding/Wash Buffer containing 20% ethanol at 2°C to 8°C. Do not freeze.

Beads Diameter

90 μm

Binding Capacity

>30 mg human IgG/mL settled resin

Ligand

Recombinant protein L produced in E.coli

Matrix

Highly cross-linked 6% agarose beads

Maximum pressure

0.3 MPa (3 bar)

Chemical stability

All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

Flow rate

Gravity flow, 100 - 300 cm/hour

pH stability

Long term: pH3 - 9;

Short term: pH2 - 10

Storage buffer

1x PBS containing 20% ethanol

市售抗体通常含有干扰酶或荧光团标记反应的物质（例如BSA、三嗪、叠氮化物）。P2710快速去除这些污染物。它还可以用于从诸如腹水或免疫血清的粗样品中纯化抗体。待纯化或净化的抗体的理想体积为100µl至0.5ml。每次运行最多可纯化500µg抗体。

该方法包括在蛋白L树脂上捕获抗体。蛋白L对来自各种物种的IgG分子的Fc区具有高亲和力。一旦抗体与L蛋白结合，就可以通过简单地清洗树脂来去除不需要的物质。每次运行最多可纯化500ug抗体。

描述

用于抗体纯化的蛋白L琼脂糖珠

经过测试的应用实验

抗体纯化

类型

10 ml 50%浆料

存储说明

在2°C至8°C下，将蛋白L树脂储存在含有20%乙醇的结合/洗涤缓冲液中。不要冻结。

beads直径

90微米

结合能力

>30 mg人IgG/mL沉淀树脂

配体

大肠杆菌中产生的重组蛋白L

基质

高交联6%琼脂糖珠

最大压力

0.3 MPa（3bar）

化学稳定性

所有常用的水性缓冲液，包括6M盐酸胍和8M尿素。

流速

重力流，100-300 cm/小时

pH稳定性

长期：pH3-9；

短期：pH2-10

存储缓冲液

1x PBS，含20%乙醇

Recombinant Protein L derived ligand

The B4 domain was code optimized and expressed as a tetramer in E. coli.