Protein A 抗体纯化磁珠

产品名称：Protein A Magnetic Beads for Antibody Purification， Protein A 抗体纯化磁珠

货号：P2611

品牌：Engibody

关键词：Protein A 抗体纯化磁珠，主要用于人源及兔源的单克隆抗体和多克隆抗体的纯化。

Protein A Magnetic Beads are used for purifying antibody from serum, cell culture supernatant or ascites, as well as for IP/Co-IP of antigens from cell or tissue extracts. Protein A can bind to antibodies from many different species, including mouse, human, rabbit, pig, dog, and cat. The protocol for the Protein A beads has been optimized to allow for high recovery and high purity of the isolated antibody or antigen. Antibody or antigen/antibody complex (IP) is first captured on the magnetic beads. The beads are washed and then the target is eluted with low pH elution buffer. IP performance is equivalent to or better than Protein A magnetic beads from other suppliers.

Description

Protein A Magnetic Beads for Antibody Purification

Tested applications

Antibody Purification

Form

2ml 25% slurry

Storage instruction

2 - 8°C

Attention: DO NOT freeze

Beads Diameter

average 40 μm

Binding Capacity

more than 10 mg Rabbit IgG per 1 ml settled beads (e.g. 4 ml 25% slurry)

Ligand

Recombinant protein A produced in E.coli

Matrix

Cross-linked agarose beaded particles containing the superparamagnetic core

Chemical stability

All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

pH stability

Long term: pH2 - 9;

Short term: pH2 - 11

Storage buffer

1x PBS containing 0.02% NaN3

Highlights

Superparamagnetic core with fast response to magnetic field

Low nonspecific binding for assay accuracy

High density of the Protein A ligand for optimal yield of antibodies and Ig fragments

High stability in common buffer systems and detergents

蛋白A磁珠用于从血清、细胞培养上清液或腹水中纯化抗体。蛋白质A可以与许多不同物种的抗体结合，包括小鼠、人、兔、猪、狗和猫。

描述

用于抗体纯化的蛋白A磁珠

经过测试的应用实验

抗体纯化

类型

2ml 25%浆料

存储说明

2-8°C

注意：请勿冻结

磁珠直径

平均40μm

结合抗体能力

每1ml沉淀珠粒（例如4ml 25%浆料）中结合纯化超过10mg兔IgG

配体

大肠杆菌中产生的重组蛋白A

基质

含有超顺磁性核的交联琼脂糖珠状颗粒

化学稳定性

所有常用的水性缓冲液，包括6M盐酸胍和8M尿素。

pH稳定性

长期：pH2-9；

短期：pH2-11

存储缓冲液

1x PBS，含0.02%NaN3

产品优势

磁场响应快的超顺磁芯

测定准确性的低非特异性结合

蛋白A配体的高密度可获得抗体和Ig片段的最佳产量

普通缓冲系统和洗涤剂的高稳定性

Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.