Protein G抗体纯化磁珠

产品名称：Protein G Magnetic Beads for Antibody Purification， Protein G抗体纯化磁珠

货号： P2712

品牌：Engibody

关键词：Protein G抗体纯化磁珠，主要用于小鼠源的单克隆抗体和多克隆抗体的纯化。

Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Description

Protein G Magnetic Beads for Antibody Purification

Tested applications

Small-scale Antibody Purification

Form

2ml 25% slurry

Storage instruction

Store at 4℃, DO NOT freeze.

Beads Diameter

average 40 μm

Binding Capacity

more than 10 mg mouse IgG per 1 ml settled beads (e.g. 4 ml 25% slurry)

Ligand

Recombinant protein G produced in E.coli

Matrix

Cross-linked agarose beaded particles containing the superparamagnetic core

Chemical stability

All commonly used aqueous buffers, including 6 M guanidine-HCl and 8 M urea.

pH stability

Long term: pH2 - 9;

Short term: pH2 - 11

Storage buffer

1x PBS containing 0.02% NaN3

Highlights

High efficiency – equivalent or higher yield of IP target antigens than magnetic beads from other suppliers

Low non-specific binding – stable, pre-blocked beads provide highly purified product (e.g., antigen eluted in IP with antibody is devoid of contaminating protein from complex cell lysate)

Consistent – magnetic beads eliminate resin loss and provide for more efficient separation than traditional IP methods that use only centrifugation

蛋白G磁珠用于从血清、细胞培养上清液或腹水中纯化抗体。蛋白质G可以与许多不同物种的抗体结合，包括小鼠、人、兔、猪、狗和猫。蛋白G磁珠的方案已被优化以允许分离的抗体或抗原的高回收率和高纯度。

产品描述

用于抗体纯化的蛋白G磁珠

经过测试的应用实验

抗体纯化

类型

2ml 25%浆料

存储说明

2-8°C

注意：请勿冻结

磁珠直径

平均40μm

结合纯化能力

每1ml沉淀珠粒（例如4ml 25%浆料）能结合纯化含有超过10mg兔IgG

配体

大肠杆菌中产生的重组蛋白G

基质

含有超顺磁性核的交联琼脂糖珠状颗粒

化学稳定性

所有常用的水性缓冲液，包括6M盐酸胍和8M尿素。

pH稳定性

长期：pH2-9；

短期：pH2-11

存储缓冲液

1x PBS，含0.02%NaN3

产品优势

磁场响应快的超顺磁芯

测定准确性的低非特异性结合

蛋白G配体的高密度可获得抗体和Ig片段的最佳产量

普通缓冲系统和洗涤剂的高稳定性

Protein A and G are popular choices for antibody purification, because they are both stable and target selective. IgG class antibodies from multiple species bind to protein A and/or G, allowing antibody to be captured on protein-bound beads. Protein A and G bind IgG subtypes with varying affinities, determined by species and the properties of the heavy chain. This chart that reports the affinity of protein A and G for various species immunoglobulin subtypes.