Ni-NTA填料（his蛋白纯化填料）

产品名称：Ni-NTA Agarose Resin 6FF for His tagged Protein Purification   Ni-NTA填料（his蛋白纯化填料）

货号： P3403

品牌：Engibody

简介：

Ni-NTA Resin,

Ni-NTA agarose,

His蛋白纯化填料

Ni-NTA Superflow sepharose is a nitrilotriacetic acid (NTA) modified Superflow 6 support charged with divalent nickel (Ni+2) designed for FPLC purification of poly-histidine-tagged proteins.

Ni-NTA Superflow sepharose is a highly crosslinked, durable resin that does not compress at flow rates used for medium- or large-scale FPLC purifications. The resin holds up well to a variety of chemicals and pH values, and is compatible with common clean-in-place procedures. Compared to cobalt and other ligands used for immobilized metal affinity chromatography (IMAC), nickel provides greater capacity for His-tagged protein purification.

Ni-NTA Superflow sepharose exhibits a high dynamic binding capacity across a range of flow rates, making it an excellent choice for larger scale purifications.

Description

Ni-NTA Agarose Resin 6FF for His tagged Protein Purification

Tested applications

immobilized metal affinity chromatography (IMAC) for His tagged Protein Purification

Storage instruction

2 to 8°C,

Up to 25 times

Conjugation

Metal: ≥15μmol Ni2+/mL resin

Beads Diameter

90 μm

Binding Capacity

Static: approx. 60mg 6xHis-GFP/mL resin

Dynamic: approx. 20mg 6xHis-GFP/mL resin

Matrix

Superflow Resin, highly crosslinked 6% sepharose

Chemical stability

1M acetic acid pH 2, 1% SDS pH 7, 6M guanidine-HCl, 70% ethanol for ≥1 week at 37°C; 8M urea, 10mM DTT, 5mM TCEP for ≥2 hours at 22°C

Flow rate

Recommended: 300cm/hour (binding, wash, elution)

Maximum: 1200cm/hour

pH stability

pH 3 to 9 for ≥1 week at 4°C

pH 2 to 3 or 9 to 12 for ≥ 2 hours at 22°C

Storage buffer

20% ethanol

Highlights

High capacity – binds greater than 20mg of His-tagged GFP per mL of resin at a linear flow rate of 300cm/hour

High purity – provides greater than 80% purity of eluted fractions when used to purify from lysates

Versatile – can be used to purify proteins under native and denaturing conditions

Robust – highly crosslinked beads tolerate linear flow rates up to 1200cm/hour

Compatible – maintains function in a wide variety of chemicals and pH conditions

Cost effective – competitively priced resin is stable through multiple cycles of cleaning and reuse

Ni-NTA Superflow sepharose是一种亚硝酸三乙酸（NTA）修饰的Superflow6载体，其填充有二价镍（Ni+2），设计用于FPLC纯化聚his标记的蛋白质。

Ni-NTA Superflow sepharose是一种高度交联、耐用的树脂，在用于中等或大规模FPLC纯化的流速下不会压缩。该树脂能很好地耐受各种化学品和pH值，并与常见的就地清洁程序兼容。与用于固定化金属亲和层析（IMAC）的钴和其他配体相比，镍为His标记的蛋白质纯化提供了更大的能力。

Ni-NTA Superflow琼脂糖在一系列流速下表现出高动态结合能力，使其成为大规模纯化的良好选择。

描述

用于His标记蛋白纯化的Ni-NTA琼脂糖树脂6FF

经过测试的应用实验

用于His标记蛋白纯化的固定化金属亲和层析（IMAC）

存储说明

2至8°C，

最多25次

偶联

镍离子：≥15μmol Ni2+/mL树脂

beads直径

90微米

结合能力

静态：约60mg 6xHis GFP/mL树脂

动态：约20mg 6xHis GFP/mL树脂

基质

Superflow树脂，高交联6%琼脂糖

化学稳定性

1M乙酸pH 2，1%SDS pH 7，6M盐酸胍，70%乙醇≥37°C下1周；8M尿素、10mM DTT、5mM TCEP≥22°C下2小时

流速

建议：300cm/小时（结合、洗涤、洗脱）

最大：1200cm/小时

pH稳定性

pH 3至9≥4°C下1周

pH 2至3或9至12≥ 22°C下2小时

存储缓冲液

20%乙醇

产品优势

高容量–以300cm/小时的线性流速，每毫升树脂结合大于20mg的His标记GFP

高纯度–当用于从裂解物中纯化时，洗脱级分的纯度大于80%

多功能–可用于在天然和变性条件下纯化蛋白质

坚固–高度交联的珠粒可承受高达1200cm/小时的线性流速

兼容–在多种化学品和pH条件下保持功能

性价比高–价格有竞争力的树脂在多次清洗和重复使用过程中保持稳定