Glutathione填料（GST蛋白纯化填料）

产品名称：Glutathione Agarose Resin 6FF for GST-Tagged Protein Purification， Glutathione填料（GST蛋白纯化填料）

货号： P3401

品牌：Engibody

关键词：GSH resin, GSH Sepharose,  Glutathione Resin, GST蛋白纯化填料，用于GST标签融合蛋白的纯化。

Glutathione agarose 6 Fast Flow is an affinity medium for fast, one-step purification of Glutathione S-Transferase (GST) tagged proteins. Provides good flow properties, excellent for scale-up.

Fast, one-step purification of GST-tagged proteins

Mild elution conditions preserving protein antigenicity and function

For scale-up due to high flow properties

Glutathione coupled to agarose 6 Fast Flow via a 10-carbon spacer arm

Glutathione agarose 6 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function. Agarose 6 Fast Flow provides good flow properties and is excellent for scaling up

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.

Description

Glutathione Agarose Resin 6FF for GST-Tagged Protein Purification

Tested applications

GST-Tagged Fusion Protein Purification

Storage instruction

2 to 8°C, 20% Ethanol

Beads Diameter

90 µm

Binding Capacity

>25 mg GST (26 kDa) /mL settled resin

Ligand

Glutathione and 10-carbon linker arm

Matrix

Highly cross-linked 6% agarose beads

Chemical stability

All commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Flow rate

<450 cm/h

pH stability

3-12

GSH琼脂糖6 Fast Flow是一种亲和纯化填料，用于GST标签融合蛋白的快速一步纯化。提供良好的流动特性，非常适合放大。

产品优点

GST标记蛋白的快速一步纯化

温和的洗脱条件保护蛋白质的抗原性和功能

用于因高流量特性而扩大规模

GSH通过10碳间隔臂与琼脂糖6 Fast Flow偶联

GSH琼脂糖6 Fast Flow用于从细菌裂解物和其他表达体系中纯化GST标签融合的蛋白。温和的洗脱条件保留了蛋白质的抗原性和功能。琼脂糖6 Fast Flow具有良好的流动性能，非常适合扩大规模

GST与GSH的结合依赖于流速，较低的流速通常会增加结合能力。在样品加载和洗脱过程中，这一点很重要。

产品描述

GSH琼脂糖树脂6FF用于GST标记蛋白纯化

经过测试的应用实验

GST标签融合蛋白纯化

存储说明

2至8°C，20%乙醇

Beads直径

90µm

结合能力

>25 mg GST（26 kDa）/mL沉降树脂

配体

GSH和10碳连接臂

基质

高交联6%琼脂糖珠

化学稳定性

所有常用的水性缓冲剂，例如1M醋酸盐，pH 6.0，0.1M NaOH，70%乙醇或6M盐酸胍，室温下1小时

流速

<450厘米/小时

pH稳定性

3-12