Dextrin填料（MBP蛋白纯化填料）

产品名称：Dextrin Agarose Resin 6FF for MBP-Tagged Protein Purification， Dextrin填料（MBP蛋白纯化填料）

货号： P4160

品牌：Engibody

关键词：Dextrin Resin, 麦芽糖结合蛋白（MBP）蛋白纯化填料，用于MBP标签融合蛋白的纯化。

Dextrin Beads 6FF is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).

Tested applications

Dextrin Beads 6FF provides convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Dextrin Beads 6FF based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.

Storage instruction

2°C to 8°C in 20% ethanol

Beads Diameter

90 µm

Binding Capacity

Approx. 7 mg MBP2*-paramyosin-δ-Sal/ml medium (Mr ~70 000,  multimer in solution)

Approx. 16 mg MBP2*-β-galactosidase/ml medium (Mr ~158 000,  multimer in solution)

Ligand

Dextrin

Matrix

Rigid, highly cross-linked 6% agarose beads

Maximum pressure

0.3 MPa, 3 bar

Chemical stability

Stable in all commonly used aqueous buffers, 0.5 M NaOH  (regeneration and cleaning)

Flow rate

Recommended flow velocity ≤ 150 cm/h, Maximum flow velocity < 300 cm/h

pH stability

working range > 7

short-term：2 to 13

Highlights

• Dextrin Beads 6FF is a robust, high resolution medium based on the 34 μm agarose matrix

• The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps

• Physiological conditions and mild elution preserve target Protein activity

• Dextrin Beads 6FF tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications

Dextrin偶联琼脂糖珠6FF是麦芽糖结合蛋白（MBP）标签融合蛋白的亲和纯化介质。

经过测试的应用

Dextrin偶联琼脂糖珠6FF提供了用麦芽糖结合蛋白（MBP）标签融合蛋白的方便亲和纯化。这种强健稳定的基于葡聚糖珠6FF的培养基能够在窄峰中洗脱目标蛋白。

存储说明

20%乙醇中的2°C至8°C

Beads直径

90µm

结合能力

约7 mg MBP2*-副肌球蛋白-δ-Sal/ml培养基（Mr~70 000，溶液中的多聚体）

约16 mg MBP2*-β-半乳糖苷酶/ml培养基（Mr~158000，溶液中的多聚体）

配体

Dextrin

基质

刚性、高度交联的6%琼脂糖珠

最大压力

0.3 MPa，3bar

化学稳定性

在所有常用的水性缓冲液中稳定，0.5M NaOH（再生和清洗）

流速

建议流速≤ 150 cm/h，最大流速<300 cm/h

pH稳定性

工作范围>7

短期：2至13

产品优势

•Dextrin偶联琼脂糖珠6FF是一种基于90μm琼脂糖基质的稳健、高分辨率介质

•大小均匀的小珠确保MBP标记的蛋白质以窄峰洗脱，从而最大限度地减少进一步浓缩步骤的需要

•生理条件和温和洗脱可保护靶蛋白活性

•Dextrin偶联琼脂糖珠6FF可耐受所有常用的水性缓冲液，并可使用0.5 M NaOH轻松再生，允许使用相同的柱进行重复纯化