Ni-NTA纯化磁珠（His蛋白纯化）

产品名称：Ni-NTA Magnetic Beads for His-tagged Protein Purification，Ni-NTA纯化磁珠（His蛋白纯化磁珠）

货号： P0030

品牌：Engibody

关键词：Ni-NTA纯化磁珠，his蛋白纯化磁珠，用于his融合蛋白的富集纯化。

Magit™ Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.

Description

Ni-NTA Magnetic Beads for His-tagged Fusion Protein Purification

Tested applications

His-tagged Fusion Protein Purification

Form

20% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol.

Storage instruction

Store at 4℃, DO NOT freeze.

Conjugation

Nickel loaded on nitrilotriacetic acid that has been covalently coupled to a blocked magnetic bead surface

Beads Diameter

average 40 μm

Binding Capacity

more than 3 to 4 mg His tagged fusion protein per 1 ml 20% slurry

Highlights

Extremely low nonspecific binding

Magnetic Beads can be regenerated for multiple uses

High capacity, equivalent or higher binding capacity than Ni-NTA magnetic beads from other suppliers

Magit™ Ni-NTA磁珠用于小规模亲和纯化以及重组His标记蛋白的高通量筛选。6xHis标签是流行的亲和标签，通常由六个连续的his残基（6xHis）组成。这些标记的蛋白质在许多不同的系统中过表达，最常见于细菌，并从细胞裂解物中纯化。使用与his侧链配位的带镍的NTA螯合物实现His标记蛋白的纯化。NTA螯合物包含四个金属结合位点，允许低金属离子浸出和高结合能力。Ni-NTA磁珠的方案已经优化，以允许分离的His标记蛋白的高纯度。性能相当于或优于其他供应商的Ni-NTA磁珠。

描述

用于His标签融合蛋白纯化的Ni-NTA磁珠

经过测试的应用

他标记的融合蛋白纯化

类型

20%的磷酸盐缓冲盐水（PBS）浆液，pH 7.4，含20%乙醇。

存储说明

储存于4℃，请勿冷冻。

偶联

负载在亚硝酸三乙酸上的镍，该镍已共价偶联到磁珠表面

磁珠直径

平均40μm

结合能力

每1ml 20%浆料中超过3-4mg His标签融合蛋白

产品优势

非特异性结合极低

磁珠可再生用于多种用途

与其他供应商的Ni-NTA磁珠相比，具有高容量、同等或更高的结合能力