DNA Pull-Down磁珠

产品名称：Streptavidin Magnetic Beads for DNA-protein pull-down assay，DNA Pull-Down磁珠

货号： IF9303

关键词： DNA Pull-Down磁珠，用于Pull-Down实验中DNA与蛋白的互作研究

Pull-down assays are used to selectively extract a protein–DNA complex from a sample. Typically, the pull-down assay uses a DNA probe labeled with a high affinity tag, such as biotin, which allows the probe to be immobilized.

Bioinylated DNA fragment containing gene promoter sequence,  immobilized to streptavidin magnetic beads, Add cell extract into beads, incubation and magnetic separation, remove non-DNA binding proteins, Elute DNA sequence specific protein Identification and characterization

Tested applications

For coupling of DNA Oligonucleotide. Optimal amount should be determined by the end user.

Specificity

Binding to biotinylated molecules, e.g. DNA Oligonucleotide.

Form

Beads in Suspension (10 mg/mL), supplied in phosphate buffered saline (PBS) pH 7.4, with 0.1% bovine serum albumin (BSA) and 0.02% sodium azide as preservatives.

Storage instruction

Store at 4°C. This product is stable for 12 months. Do not freeze, dry or centrifuge beads. This may cause irreversible aggregation and decreased binding capacity.

Bead Diameter:1 µm

Concentration:10 mg/mL

Binding Capacity:>100nmol Free biotin/mL beads slurry

下拉分析用于从样品中选择性提取蛋白质-DNA复合物。通常，下拉分析使用标记有高亲和力标签（如biotin）的DNA探针，这允许探针被固定。

含有基因启动子序列的biotin化DNA片段，固定在链霉亲和素磁珠上，将细胞提取物加入磁珠中，温育和磁分离，去除非DNA结合蛋白，洗脱DNA序列特异性蛋白鉴定和表征。

经过测试的应用

用于DNA寡核苷酸的偶联。最佳用量应由最终用户确定。

特异性

与biotin化分子结合，如DNA寡核苷酸。

类型

悬浮磁珠（10 mg/mL），在pH 7.4的磷酸盐缓冲盐水（PBS）中提供，0.1%牛血清白蛋白（BSA）和0.02%防腐剂。

存储说明

储存于4°C。该产品稳定12个月。请勿冷冻、干燥或离心珠粒。这可能导致不可逆聚集和结合能力降低。

磁珠直径：1µm

浓度：10 mg/mL

结合能力：>100nmol游离biotin/mL珠浆

Figure 1. DNA Oligonucleotide pull-down assay for promoter binding transcription factors. A lysate is made of isolated nuclei from bone marrow-derived macrophages (here the macrophages were activated with the cytokine interferon gamma (IFNg)). To this lysate, the biotinylated custom oligo is added. The 20mer oligo corresponds to the promoter of a gene of interest; in this case the gene was inducible nitric oxide synthase (iNOS). Transcription factors bind the biotinylated oligo and these protein:DNA complexes are then isolated using streptavidin magnetic beads. The complexes are then subjected to SDS-PAGE and Western blotting, in this example, for the transcription factor STAT1. STAT1 translocates to the nucleus with interferon gamma treatment (top panel (+)) and is phosphorylated/activated (middle panel (+)). The pull down panel (bottom) demonstrates that in response to IFNg treatment STAT1 binds to the iNOS promoter.