His 蛋白纯化试剂盒(磁珠法)

产品名称：His tagged Protein Purification Kit (Ni-NTA Magnetic Beads, Soluble Protein)

品牌：Engibody

货号：P5601

简介：His 蛋白纯化试剂盒(磁珠法)，用于His标签融合蛋白的富集纯化

Ni-NTA Magnetic Beads are used for small scale affinity purification as well as high-throughput screening of recombinant His-tagged proteins. The polyhistidine tag is the most popular affinity tag and typically consists of six consecutive histidine residues (6xHis). These tagged proteins are overexpressed in a number of different systems, most commonly in bacteria, and purified from cell lysates. Purification of His-tagged proteins is achieved using a NTA chelate charged with nickel that coordinates with the histidine side chains. The NTA chelate contains four metal-binding sites which allow for low metal ion leaching and high binding capacity. The protocol for the Ni-NTA Magnetic Beads has been optimized to allow for high purity of the isolated His-tagged protein. Performance is equivalent to or better than Ni-NTA magnetic beads from other suppliers.

Description

His tagged Fusion Protein Purification Kit (Ni-NTA Magnetic Beads, Soluble Protein)

Tested applications

His-tagged Protein Purification ( by use Ni-NTA Magnetic Beads)

Components

Ni-NTA Magnetic Beads: 1ml

5g NiSO4;  

6g imidazole;  

10g EDTA

Binding Buffer: 100ml

Wash BufferL: 200ml

Storage instruction

2 - 8°C, do not freeze

Ni-NTA磁珠用于小规模亲和纯化以及重组His标记蛋白的高通量筛选。聚his标签是的亲和标签，通常由六个连续的（6xHis残基）组成。这些标签融合的蛋白质在许多不同的系统中过表达，最常见于细菌，并从细胞裂解物中纯化。使用与histidine侧链配位的带镍的NTA螯合物实现His标记蛋白的纯化。NTA螯合物包含四个金属结合位点，允许低金属离子浸出和高结合能力。Ni-NTA磁珠的方案已经优化，以允许分离的His标记蛋白的高纯度。性能优于其他供应商的Ni-NTA磁珠。

产品描述

his标签融合蛋白纯化试剂盒（Ni-NTA磁珠，可溶性蛋白）

经过测试的应用

his标签融合蛋白纯化（通过使用Ni-NTA磁珠）

Kit内组份

Ni-NTA磁珠：1ml

5克硫酸镍；

6克咪唑；

10克EDTA

Binding缓冲液：100ml

Wash缓冲液L:200ml

存储说明

2-8°C，请勿冷冻

仅用于科研，不作用人体